vnmrs 600 mhz spectrometer  (Varian Medical)

Journal: Protein Science : A Publication of the Protein Society

Article Title: Functionally related internal fluctuations in human ileal bile acid‐binding protein by high pressure nuclear magnetic resonance

doi: 10.1002/pro.70608

Figure Lengend Snippet: Stacked‐plot of one‐dimensional 1 H‐NMR spectra of hI‐BABP (0.4 mM, pH = 6.3) recorded as a function of pressure (18°C, 600 MHz). Regions corresponding to the amide HN and the methyl resonances are highlighted. The decrease with pressure of the W49 side chain resonance corresponding to the folded state (10.72 ppm) with a concomitant appearance of a new peak at ~2000 bar representing the unfolded state (10.06 ppm) is shown in large in the left. Note the intermediate resonance between the folded and unfolded peaks of W49 sc (red dot). (The peak at 10.27 ppm corresponds to the backbone amide of E9.)

Article Snippet: One‐dimensional (1D) 1 H (Scott et al., ) and two‐dimensional (2D) 1 H‐ 15 N heteronuclear single quantum coherence (HSQC) (Kay et al., ) experiments were carried out on a 600 Varian NMR SYSTEMTM spectrometer equipped with a 5‐mm indirect detection triple ( 1 H 13 C 15 N) z‐axis gradient probe at 18°C at the following pressures: 1, 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, and 3000 bar.

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